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Tuesday, April 28, 2020 | History

5 edition of Long-term bone marrow culture found in the catalog.

Long-term bone marrow culture

proceedings of a symposium held at the Kroc Foundation, Santa Ynez Valley, California, September 12-16, 1983

by

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  • 7 Currently reading

Published by A.R. Liss in New York .
Written in English

    Subjects:
  • Hematopoiesis -- Congresses.,
  • Bone marrow -- Congresses.,
  • Tissue culture -- Congresses.,
  • Bone Marrow -- congresses.,
  • Tissue Culture -- methods -- congresses.,
  • Hematopoiesis -- congresses.

  • Edition Notes

    Includes bibliographies and index.

    Statementco-sponsored by the National Heart, Lung, and Blood Institute, National Institutes of Health, and the Kroc Foundation, editors, Daniel G. Wright, Joel S. Greenberger.
    SeriesKroc Foundation series ;, v. 18
    ContributionsWright, Daniel G., Greenberger, Joel S., National Heart, Lung, and Blood Institute., Kroc Foundation.
    Classifications
    LC ClassificationsQP92 .L66 1984
    The Physical Object
    Paginationxiv, 440 p. :
    Number of Pages440
    ID Numbers
    Open LibraryOL2854684M
    ISBN 100845103083
    LC Control Number84017148
    OCLC/WorldCa11068916


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Long-term bone marrow culture Download PDF EPUB FB2

Here, in a subsequent protocol, we describe an improved bone marrow culture system yielding a reliable growth of bone marrow cells and leading to a greater clinical response.

Cells expressing markers of endothelial progenitors including CD, CD, and. Distinct Requirements for Optimal Growth and In Vitro Expansion of Human CD34+CD38− Bone Marrow Long-Term Culture-Initiating Cells (LTC-IC), Extended LTC-IC, and Murine In Vivo Long-Term.

Human Long-Term Bone Marrow Culture. Abstract. The bone marrow is the primary site of hematopoiesis in adults.

Accurate in vitro models of hematopoietic regulation and function should reflect the various hormonal and environmental regulators that act on developing hematopoietic cells in the by: Long-term liquid culture systems for the cultivation of human bone marrow cells are urgently needed.

Moore and Sheridan () reported the establishment of human marrow cultures using conditions similar to those described by Dexter et al., but CFUc pro duc­ tion was limited to weeks.

More recently. Long-Term Human Bone Marrow Cultures Blood. Vol. 56, No. 1 (July), By William G. Hocking and David W. Golde. The effect of stem cell factor (SCF) on the establishment of hematopoietic activity in murine long-term bone marrow cultures (LTBMC) was investigated by addition of SCF to (a) normal LTBMC from the onset of culture and (b) pre-established irradiated bone marrow stroma inoculated with lineage negative (Lin-primitive hematopoietic progenitor cells enriched on the basis of low rhodamine Cited by: 6.

Blood culture. Bone-marrow culture is the most reliable method for the diagnosis of enteric fever. The organisms may also be recovered from the bloodstream at any stage of the illness, but are most commonly found during the first 7–10 days and during relapses.

Abstract. Growth of mature B cells and their precursors from mouse bone marrow was maintained in culture for greater than 1 year. Feeder layers of adherent bone marrow cells, established in medium containing fetal calf serum and no exogenous steroids, were used to provide an in vitro environment that supported continuous growth and development of these by: Coulombel, L., Eaves, A.

C, and Eaves, C. () Enzymatic treatment of long-term marrow cultures reveals the preferential location of primitive hemopoietic progenitors in the adherent layer. Bl Cited by: Long-term bone marrow culture is applied in fundamental studies of normal and pathologically altered hematopoiesis, in pharmacological research, in the purging of residual leukemia cells from bone marrow autotransplants, and in the gene : Silvana Stanović and Milivoj Boranić.

A method has been described for the long-term culture of human bone marrow cells in liquid medium. Hematopoiesis, as measured by the production of granulocytic-macrophage progenitor cells (CFUc), continued for at least 20 weeks and was dependent upon the presence of a marrow-derived Cited by:   A method has been described for the long-term culture of human bone marrow cells in liquid medium.

Hematopoiesis, as measured by the production of granulocytic-macrophage progenitor cells (CFUc), continued for at least 20 weeks and was dependent upon the presence of a marrow-derived adherent layer of by:   The proliferation of multipotential haematopoietic stem cells (CFU-S) is possible in some long-term bone marrow cultures 1–ocyte and macrophage progenitors (CFU-C) 2 and megakaryocyte Cited by: In vitro data from supernatants of long-term bone marrow cultures suggest that marrow stromal cells produce reduced levels of IL-3 in patients with aplastic anemia.

1 IL-3 has also been implicated in patients with acute lymphocytic leukemia (ALL) with a ()(q31;q32) translocation. 1 In two such patients, the translocation resulted in juxtaposition of the IL-3 gene and the Ig heavy-chain gene, and. Long-Term Bone Marrow-Derived Cultures. Mice were euthanized, and bone marrow was harvested by flushing the femurs and tibias with LTBMC media (79% by volume Fischer’s medium, 20% horse serum, 1 μM hydrocortisone, 2 mM L-glutamine.

Get this from a library. Long-term bone marrow culture: proceedings of a symposium held at the Kroc Foundation, Santa Ynez Valley, California, September[Daniel G Wright; Joel S Greenberger; National Heart, Lung, and Blood Institute.; Kroc Foundation.;].

For example, bFGF was used in a combination with bone-marrow-conditioned medium, and hypoxia to expand BMSCs in long term culture and to maintain their stemness.

The action of FGF to inhibit cellular senescence and to promote proliferation of mouse BMSC was found to be mediated through the suppression of PI3K/AKT-MDM2 pathway [ 27 ] and Cited by: 1. The maintenance of hemopoietic precursors in long‐term liquid bone marrow cultures (LTBMC) is associated with the presence of an adherent stromal layer composed of heterogeneous cell populations.

We have used a culture assay to promote the growth of Cited by: Marrow specimens were part of aspirates taken for diagnostic purposes andwereobtainedwith informed consent. Eachaspirate was collected in a sterile tube containing units of preservative-free heparin. Long-term cultures. The procedure used to initiate and maintain long-term marrowcultures wasthe same as that described previously (10).

OBJECTIVES. This in vitro study was carried out to define the best experimental conditions for producing canine neutrophils in long-term bone marrow culture (LTBMC), to determine the functional parameters of neutrophils obtained from peripheral blood and LTBMC and to ascertain whether these cells display physiological similarities.

Our aim is to provide an experimental model enabling. Novel three-dimensional long-term bone marrow culture system using polymer particles with grafted epoxy-polymer-chains supports the proliferation and differentiation of hematopoietic stem cells Yukio Hirabayashi, Yoshihiro Hatta, Jin Takeuchi, Isao Tsuboi, Tomonori Harada, Kentaro Ono, Wilhelm Robert Glomm, Masahiro Yasuda, and Shin AizawaCited by:   Long-term Preservation of Bone Marrow.

During the course of experiments with various techniques for freezing and storing mouse bone marrow 3, we froze a Cited by: 4. Bone marrow culture is an examination of the soft, fatty tissue found inside certain bones.

The bone marrow tissue produces blood cells. This test is done to look for an infection inside the bone marrow. The doctor removes a sample of your bone marrow from the back of. Megakaryocytic maturation was analyzed in long-term bone marrow cultures in the absence of added growth factors.

Megakaryocytes could be observed for periods of up to 13 weeks in both the supernatant and stromal layer of these cultures. NK cells from long-term bone marrow culture (LTBMC) were compared with ILactivated splenic NK cells [short-term spleen cell culture (STSC)] with r We use cookies to enhance your experience on our continuing to use our website, you are agreeing to our use of by: related.

The list of acronyms and abbreviations related to LTC - Long-term bone marrow culture. The Effect of Bone Marrow Fibroblast and Stromal Cell-Conditioned Media on Hemopoietic Cells in Culture A. Zaritskey and O.

Strizhak 1 Introduction It is well known that fibroblasts [1] and stromal cells (Sc) are the main compo­ nents of the bone marrow microenviron­ ment [2. A mathematical model of mouse granulopoiesis in long‐term bone marrow culture was constructed, based on established in vivo cell kinetic parameters.

We applied the model to the cell kinetic experiment presented in Part I. Comparing model‐predicted cell kinetics with the experimental data led to iterative testing of several by: 4. CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): The strong and long-lasting hematotoxic effect after benzene exposure in vivo ( ppm, 6 hr/day, 5 days/week for 2 weeks) was assessed in mice with bone marrow cells grown in longterm bone marrow culture (LTBMC).

Bone marrow cultures initiated 1 day after the last benzene exposure did not produce adequate. Long-term culture initiating cell (LTC-IC) assay. A modified LTC-IC assay was performed as previously described.

Briefly, irradiated (80 Gy) mouse bone marrow stromal cells (MB4, American Type Culture Collection, Rockville, MD, USA) were seeded at 10 5 cells/well in well flatCited by: Mouse models of eosinophil-associated diseases have been used to study the mechanisms of disease pathogenesis.

In this study, mouse-derived bone marrow cells were used in long-term (6 and 9 months) cell cultures of differentiated eosinophils and macrophages. IL-5 was used to differentiate the stem cells to eosinophils and GM-CSF was used to propagate macrophages from the bone marrow stem : Olena B.

Svitlova. Assessed by Long-term Bone Marrow Culture Nadar G. Abraham The Rockefeller University, New York, New York The strong and long-lasting hematotoxic effect after benzene exposure in vivo ( ppm, 6 hr/day, 5 days/week for 2 weeks) was assessed in mice with bone marrow cells grown in long-term bone marrow culture (LTBMC).

Moreover, depletion of CD + MΦ from long-term Dexter culture resulted in a 42% reduction in the ability of BM stromal cells to produce CXCL Thus, CD + MΦ in the BM promote stromal production of CXCL12, and their specific depletion in vivo is sufficient to mobilize HSCs/ by: ABSTRACTMobilized peripheral blood hematopoietic progenitor cells obtained from cancer patients treated with high-dose cyclophosphamide (7g/m2) followed by G-CSF, GM-CSF, IL-3, PIXY, or combinations of these cytokines have been successfully used.

However, fluorescence- activated cell sorted CD34+ subpopulations contain not more than 1% to 5% primitive progenitors capable of initiating and sustaining growth in long-term bone marrow culture initiating cells (LTBMC-ICs).Cited by: The spontaneous stratification in long‐term bone marrow cultures was illustrated and quantified.

The cultures were separated into three hematopoietic layers: nonadherent cells in the supernatant medium, lightly adherent cells on top of the stromal layer, and remaining cells buried within the stromal by: 4.

The strong and long-lasting hematotoxic effect after benzene exposure in vivo ( ppm, 6 hr/day, 5 days/week for 2 weeks) was assessed in mice with bone marrow cells grown in long-term bone marrow culture (LTBMC).

Bone marrow cultures initiated 1 day after the last benzene exposure did not produce adequate numbers of hematopoietic cells over 3 Cited by: Stromal cell lines, designated MS-1, -2, -3, -4, -5, -6, and -7 were established by irradiating the adherent cells in long-term bone marrow cultures with rad x.